Estrogen may offer protection against delirium SciReports
. After 3 days, mice were euthanized by perfusion, while deeply anesthetized with a combination of ketamine and dexmedetomidine, followed by a physical method for tissue and blood collection, and the brain was aseptically removed. Plasma collected at the time of tissue harvest was utilized for the quantification of IL-6 and IL-6R.Mice were deeply anesthetized and perfused with room temperature PBS with 0.5 mM ethylenediaminetetraacetic acid .
2 epi-fluorescence microscope equipped with standard filter sets/mercury arch lamp, an Apotome 2.0, and an Axiocam HRm camera controlled by Zen Blue Pro software was used to acquire and process images. Images of damage marker staining were acquired with a 10× objective as a 5 × 5 tiled image that encompassed the frontal cortex and hippocampus of each section. All acquisition and display settings are the same between groups and settings were optimized using the UTI group.
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