Nature research paper: A brainstem map for visceral sensations
mice were anaesthetised . Stomach and duodenum were exposed through a small abdominal incision. For the stomach, approximately 40 injections ofwere made into the glandular stomach wall using a Hamilton syringe. For the duodenum, approximately 5 injections ofwere made into the first 1–1.5 cm of the duodenum using a Nanoject III injector . The larynx was exposed through a small midline incision on the ventral side of the neck, and the sternohyoid muscle was retracted.
Two-photon calcium imaging was performed with an Olympus FVMPE resonant-scanning two-photon microscope equipped with a piezoelectric objective-stepper and an Olympus ×25 water-immersion objective . Olympus FluoView software was used to collect two-photon images.
. A small rodent feeding needle was affixed inside the balloon and connected to a syringe by silicon tubing, which enabled precise volume control by liquid infusion. Distension of the oral cavity was achieved by inflating a balloon placed in the mouth against the cranial base. For gastric distension, the content of the stomach was removed, and a small incision was made in the greater curvature of the glandular stomach.
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