A patient-designed tissue-engineered model of the infiltrative glioblastoma microenvironment - npj Precision Oncology

3D model of brain tumor environment could aid personalized treatment nresearchnews

A Human XL Cytokine 44-plex array was used to quantify cytokines in different cell-laden hydrogel conditions. Static hydrogel cultures were set up as described above for conditions of GSCs only , GSCs plus astrocytes, and microglia, or only astrocytes and microglia . The gels were solidified and cultured in Astrocyte basal medium +N2 +B27 without vitamin A.

We then used our model to interrogate the role of these cytokines and therefore glioma-glial signaling in glioma cell metrics. We added either cytokine neutralizing antibodies or receptor antagonists directly into the medium and pre-hydrogel solution of G34 + glial cell cultures. Neutralizing antibodies included 0.2 µg/mL α-CXCL8 , 2 µg/mL α-CCL2 , and 7 µg/mL α-CXCL1 . Appropriate antibody isotypes were used as controls.

. Animals are assessed daily for signs of distress and are euthanized accordingly when they display humane endpoint criteria.For analyses of GSC phenotype in xenograft models, the tissue samples are collected, soaked in sucrose, cryoembedded, and sectioned at 12 μm using a Leica CM 1950.

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