A study in Nutrition & Metabolism discusses a new therapeutic approach involving the transdermal administration of MC903—a synthetic derivative of vitamin D3—for the treatment of obesity and diabetes.
3T3L1 preadipocytes were differentiated into mature adipocytes, as described previously []. In brief, differentiation was induced in confluent cells with differentiation medium containing 10% fetal bovine serum , 250 nM dexamethasone, 0.5 mM isobutyl methylxanthine, and 500 nM insulin. After 3 days, the differentiation medium was replaced with post-differentiation medium containing 10% FBS and 500 nM insulin.
]. Cells were differentiated in the absence or presence of 300 nM MC903 or 1,25-dihydroxy-VitD3 for 72 h, harvested, and subjected to a real-time PCR analysis.cells/well in a Seahorse XFe24 assay plate were incubated with DMEM supplemented with 10% FBS and antibiotics. After 24 h incubation, culture media were replaced to myotube differentiation medium. Further after 24 h, medium was replaced to Opti-MEM with 0.1% FBS, and cells were incubated with lipid complexes containing 0.
Eight-week-old mice were maintained with standard chow or 60% HFD, and the MC903 treatment to the ear auricle was initiated when mice were 15 weeks old. The body weight transition in each group is shown in Fig.B. Increases in body weight were markedly higher in HFD mice than in Chow mice, and were significantly reduced by the transdermal MC903 treatment. In contrast, MC903 did not affect the body weight of Chow mice.
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