A study published in the Journal of Neuroinflammation provides unique insight into the development of human microglia and a useful reference for understanding microglial contribution to developmental and age-related human disease.
]. Briefly, histograms were plotted using the regulon activity matrix , in which columns represent cells and rows represent the AUC regulon activity and the targetome of individual TFs, both which were queried using the regulon data frame .The average expression of microglia genes from each developmental stage was measured using the “AverageExpression” function in Seurat.
Slides were imaged using a Zeiss Axio Observer at 20× for representative images, and 40× for corrected total cell fluorescence quantification. In short, CTCF values were generated by manually segmenting microglia three separate times to obtain average total microglial area, mean intensity, and integrated density. Adjacent background selections of the same area as each combination of selected microglia were also taken simultaneously for comparison.
To study changes in the transcriptional profile of microglia over time, human brain samples were collected at different developmental stages. Pre-natal samples were obtained from second trimester pre-natal brain tissue (Fig.
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