Artificial viral vectors constructed from bacteriophage T4 show promise for advanced gene delivery AVVs ArtificialViralVectors GeneTherapies PersonalizedMedicine Bacteriophage T4 Genome MolecularRepairs Nanomaterials NatureComms
By Bhavana KunkalikarJun 1 2023Reviewed by Benedette Cuffari, M.Sc. In a recent study published in the journal Nature Communications, researchers present a method for constructing artificial viral vectors using the established structural components of bacteriophage T4.
T4 is a highly efficient virus with an almost 100% infection rate and a rapid replication cycle of approximately 20-30 minutes/cycle. These characteristics indicate that T4 is an excellent foundation for constructing AVVs. RNA, proteins, and their complexes are attached to packaged head particles through highly antigenic outer capsid and small outer capsid protein interactions.
On average, each nanoparticle contained approximately five molecules of luciferase plasmid and green fluorescent protein reporter plasmid. GFP fluorescence was used to determine the transduction efficiency, which was about 100%. Genome editing AVVs Different genome editing AVVs were created by combining all editing molecules into a single AVV in various arrangements. The first set of AVVs contained plasmids with expressible Cas9 and gRNA genes, in which the Cas9 N-terminus was fused with the nuclear localization sequence PKKKRKV71 and codon-optimized to create NLS-Cas9.
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