Targeted depletion of selected gut bacteria using bacteriophages enables functional studies of the mouse microbiota. NBThighlight
culture at OD600 0.4 at 37°C with shaking until the solution became clear. The lysates were centrifuged at 4,000 × g for 30 min to remove the remaining bacterial cells and debris, and then supernatant was filtered using a 0.2 μm membrane filter and kept at 4°C. The bacteriophage titer was measured using the double-layer plate method and diluted to the required titer.
All mice were housed in the testing room for acclimation at least 1 h before behavioural testing. The animal was placed individually in the centre of the device that was constructed from an opaque black box and allowed to explore freely for 30 min. The total distance travelled and the time spent in the central zone were recorded and analysed using the SMART 3.0 software. After each mouse was tested, 75% ethanol was used to clean faeces and eliminate odour.
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