How effectively antimicrobial blue light (aBL) inactivated dried cells and biofilms.
By Neha MathurOct 19 2023Reviewed by Lily Ramsey, LLM In a recent article published in Applied and Environmental Microbiology, researchers evaluated how effectively antimicrobial blue light inactivated dried cells and biofilms of Listeria monocytogenes , a lethal foodborne pathogen.
Most previous studies have reported the effectiveness of aBL against Lm in aqueous systems. However, data are scarce on its effects against Lm-dried cells and biofilms deposited on inert surfaces, primarily stainless steel . The research team introduced 100 µL of the mixed strain cocktail onto the SS coupons and subsequently dried them in a biosafety cabinet for up to two hours to obtain dry cells. This process occurred in two series: one included the application of a photosensitizer, while the other did not. Additionally, biofilms were subjected to photosensitization tests.
Irradiation at higher wavelengths of 420 and 460 nm did not result in >2 log reductions in Lm cells exposed to EDs of ≤1,000 J/cm2 within the same time. On the contrary, irradiation at 405 nm aBL achieved the highest reductions in viability of dried Lm cells, including in biofilms. Consistent with previous findings, in this study, exposing Lm in biofilms for four hours reduced biofilm biomass regardless of the aBL wavelength applied.
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