Cell-type-specific prediction of 3D chromatin organization enables high-throughput in silico genetic screening
In silico genetic screen at 2-Mb windows
. Briefly, for each chromatin remodeling gene, we designed on average 6–8 single guide RNAs , for a total of2,500 sgRNAs. The sgRNA sequences were synthesized by Twist Bioscience, and cloned into a lentivirus-based sgRNA vector tagged with GFP . Cas9-expressing T-ALL cell lines were transduced with sgRNA library virus at a low multiplicity of infection , followed by infection efficiency assessment through GFP percentage on Day 4 posttransduction.
. For pooled CRISPR screening analysis, samples of each time-point were normalized as sgRNA read count/total read count × 100,000. Subsequently, normalized reads were then used to calculate logfc for each gRNA. The fold changes between Day 4 and Day 20 for each gene were averaged from all CRISPR gRNA targets.-test comparing the fold changes of all gRNA targets of the same gene with fold change of 1.was used to compute the interactions of each selected viewpoint in a roll-window fashion.
Ireland Latest News, Ireland Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
Programmable A-to-Y base editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase - Nature BiotechnologyProgrammable A-to-Y base editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase
Read more »
Photonic flatband resonances for free-electron radiation - NatureA study demonstrates full energy–momentum matching, and enhanced interaction, between free electrons and photons through a continuum of flatband resonances, realized in a silicon-on-insulator photonic crystal slab.
Read more »
The tragic life of lawyer who\u00a0smuggled cell phone into jail for Art Schlichter'Art Schlichter was her downfall.' Following the news this week that Art Schlichter could be heading back to prison, a look back at Dana Hunsinger Benbow's powerful story on his role in one woman's downfall.
Read more »
Inside Brock Bowers’s Meteoric Rise to Tight End Force of NatureShowing up to a 2019 camp, Brock Bowers had just one scholarship offer. RossDellenger on how in a matter of years, he became one of college football’s best tight ends
Read more »
Solar-to-hydrogen efficiency of more than 9% in photocatalytic water splitting - NaturePhotocatalytic water splitting with a high solar-to-hydrogen efficiency of more than nine per cent is achieved using pure water, concentrated solar light and an indium gallium nitride photocatalyst.
Read more »