Nature research paper: Work in fission yeast unites two opposing views of cell cycle control
. Unless otherwise stated, all experiments were conducted in yeast extract medium supplemented with adenine, leucine, histidine and uracil to a final concentration of 0.15 g l. Cells were grown at 25 °C unless stated otherwise. All experiments were performed with cells in exponential growth, defined as 2.5–10 × 10. To induce expression from the tetracycline-dependent promoter, anhydrotetracycline hydrochloride dissolved in DMSO was added to a final concentration of 0.3125 μg ml.
To check for nuclear enrichment of synCut3–mCherry and cyclin–Cdk1–sfGFP, the mean pixel value of a circle encompassing the nucleus was compared to the mean pixel value of a circle of equal area drawn in the cytoplasm. If the nuclear mean value was 1.5 times the cytoplasmic value or greater, this was classed as nuclear enrichment. Scoring for nuclear enrichment indices, cell cycle progression indices and spindle formation indices was conducted on 100 cells per time point.
Ireland Latest News, Ireland Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
How To Do High Knees For A Fiery Core & Cardio WorkoutHere's exactly how to fit high knees into your workout routine, whether it be in between sets for a quick boost of movement or within a HIIT session to challenge your cardiovascular strength. 🔥
Read more »
Analysis: Graphic video of group attack on women shakes China to the coreShocking Surveillance footage of men attacking women in northern China has rekindled a debate about violence against women and gender inequality in the country of 1.4 billion people.
Read more »
Continuous Bose–Einstein condensation - NatureContinuous, indefinitely lasting Bose–Einstein condensation, sustained by amplification through Bose-stimulated gain of atoms from a thermal bath, creates a continuous-wave condensate of strontium atoms.
Read more »
A novel study suggests that Earth's inner core oscillatesThe University of Southern California scientists have shown that the Earth’s inner core oscillates. This finding may also help explain the variation in the length of a day.
Read more »
Nation's No. 1 team repeats: No one could beat this Roncalli team. Not even Mother Nature.'It was just really a magical night,' coach David Lauck said following Roncalli's record-setting win over Harrison in the 4A championship game.
Read more »