Nature research paper: Decoding gene regulation in the fly brain
. Locus of marker genes showing differential peaks between T4 and T5 neurons and between a/b and c/d subtypes .. Experimental overview: GH146 was used to drive GFP expression in OPNs, followed by FACS and scATAC-seq.. Visualization of gene accessibility near OPN markers, showing heterogeneity between clusters.. 17k peaks were identified in the OPNs, of which 4.5k are unique, and of which 876 are near OPN marker genes . These peaks were not found in the consensus peaks as shown in the tracks..
The classifier also included the progenitor cell types –from the developmental analysis– .. Chromatin landscape for T1 neurons, shows a highly dynamic opening and closing of peaks during development. A core-set remains accessible at all times, of which a subset is specific to T1 neurons.. Bar plot showing the number of core-regions identified per cell type. Dark colours show specific core-regions .. Number of DARs calculated per cell type for every timepoint, shows a decline over time.
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