Research published in Fluids and Barriers of the CNS suggests that fasudil is a blood brain barrier-protective agent against acute ischemic stroke.
ERS-2 Voltohmmeter . The extracellular matrix-coated Millicell inserts were placed in a 24-well plate containing culture medium and were then used to measure the background resistance. The resistance measurements of blank inserts were subtracted from TEER values of inserts with cells. Values are given as Ω × cmTransendothelial permeability
The permeability of sodium fluorescein across the endothelial monolayer was determined. Cell culture inserts were transferred to 24-well plates containing 0.9 mL assay buffer with calcium and magnesium supplemented with 4.5 g/L glucose and 10 mM HEPES, and adjusted to pH 7.4) in the lower compartments. In the inserts, the culture medium was replaced with 0.2 mL assay buffer containing 10 µg/mL Na-F .
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