Research published in BMC Complementary Medicine and Therapies found that Salvia officinalis (common sage) extract has anti-inflammatory effects across multiple human cell lines.
extract were measured using the Folin and Ciocalteu method against a tannic acid standard, and total flavonoid levels were measured using the aluminium chloride method against a rutin standard, both based on three technical replicates from the protocols described by Thangaraj et al. []. Rosmarinic acid and 1,8-cineole levels were measured by GCMS analysis at RSSL .
Primary human brain microvascular cells were provided by Innoprot Spain and have been developed by immortalizing primary human brain endothelial cells with Lenti-SV40 Lentivirus. Cells were delivered at a cell density of 1 × 10and cultured in Endothelial cell medium . The media was supplemented with 5% FBS , Antibiotic-Antimycotic solution and Endothelial cell growth supplement . Media, FBS and supplements were also sourced from Innoprot, Spain.
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Breastfeeding and overweight/obesity among children and adolescents: a cross-sectional study - BMC PediatricsBackground Overweight and obesity are major public health crises among children and adolescents and contribute to a significant economic burden. We aimed to investigate the relationship between breastfeeding duration and overweight and obesity in children and adolescents in Qingdao, China in 2017. Methods This study conducted a survey with 10,753 students (5370 boys and 5383 girls) aged 6 to 16 years from the Shibei District of Qingdao, China in 2017. Anthropometric measurements were taken by well-trained personnel and self-completed questionnaires were used to collect data from students. A variety of statistical methods were used in this study, including univariate and multivariate analyses, as well as linear and nonlinear regression models. Results The prevalence of overweight and obesity was 15.45% and 19.76%, respectively. There was a significant negative correlation between breastfeeding duration and BMI in children and adolescents (β = -0.025, 95% CI: -0.033, -0.005, P | 0.01). Among boys, the BMI in children and adolescences of those who have been breastfed for more than 12 months was significantly lower than that of others whose breastfeeding duration was less than 12 months (β = -0.440, 95%CI -0.655, -0.224, P | 0.01). Breastfeeding has a particularly positive effect on the prevalence of obesity in boys aged 9 to 11 years (OR = 0.978, 95% CI: 0.958,0.999, P | 0.05). Conclusion Breastfeeding can significantly reduce the prevalence of overweight and obesity among children and adolescents aged 6 to 16 years. Those who were breastfed for more than 12 months had a lower risk of developing overweight and obesity, especially boys between the ages of 9 and 11.
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Full Monty actor Hugo Speer sacked from Disney reboot after 'inappropriate conduct' allegationsFull Monty actor Hugo Speer sacked from Disney reboot after 'inappropriate conduct' allegations. 🔴 Disney+ said it decided to terminate his contract with immediate effect after carrying out an investigation.
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BBC Confirms Six Complaints Against DJ Tim WestwoodThe BBC previously denied evidence of any complaints.
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Researchers investigate prevention and therapy against monkeypox infectionResearchers investigate prevention and therapy against monkeypox infection UofMaryland Monkeypox Smallpox Treatment Prevention MonkeypoxPrevention
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SARS-CoV-2 productively infects human brain microvascular endothelial cells - Journal of NeuroinflammationBackground The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood–brain barrier (BBB). Methods RNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes. Results A total of 927 differentially expressed genes were identified, 610 of which were significantly upregulated while the remaining 317 were downregulated. We verified the significant induction of cytokines, chemokines, and adhesion molecules in hBMECs by SARS-CoV-2, suggesting an activation of the vascular endothelium in brain. Moreover, we demonstrated that SARS-CoV-2 infection could increase the BBB permeability, by downregulating as well as remodeling the intercellular tight junction proteins. Conclusions Our findings demonstrated that SARS-CoV-2 infection can cause BBB dysfunction, providing novel insights into the understanding of SARS-CoV-2 neuropathogenesis. Moreover, this finding shall constitute a new approach for future prevention and treatment of SARS-CoV-2-induced CNS infection.
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