In this study, Cell Microsystems details how their CellRaft AIR System can be used with fluorescence activated cell sorting to accelerate the generation of single cell clones.
Sponsored Content by Cell MicrosystemsJul 12 2023Reviewed by Louis Castel Clustered Regularly Interspaced Short Palindromic Repeats , genome engineering of cell lines, facilitate target discovery. It provides an excellent disease model for preclinical research, particularly in chimeric antigen receptor T-cell therapy. Current approaches range from manual limiting dilution to a combination of high-speed cell sorters and bulk sorting followed by plating into single cells.
Leveraging Cell Microsystems' CellRaft AIR™ imaging and isolation system, the researchers collected nearly 400 rafts containing single-cell-derived clones from each array. Over 60% of these clones exhibit potential for outgrowth. However, the kinetics and the number of clones required to be tested will vary based on cell type and transfection efficiency.
The instrument requires no start-up or shut-down time. This system streamlines the consolidation of clones from multiple plates, minimizing several steps. Acknowledgments The authors of the original poster are Sobha Thamminana, Aronpreet Atwell, Jessica Hartman, Richard Smith and Bhargavi Rajan. About Cell Microsystems Making innovative tools that enable researchers to image, identify, and isolate viable single cells and clonal colonies Researchers worldwide in the fields of CRISPR gene editing, oncology, stem cell biology, immunology and neurobiology use Cell Microsystems products, advancing sophisticated discovery across the life sciences.
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