Four activists glued their hands to the frame of the Renaissance painting in the Piccadilly gallery.
The Last Supper shows the famous scene where Jesus eats with his 12 apostles for the final time and warns one will betray him.
It is believed to have been painted by Giampietrino, a student of Leonardo Da Vinci, copying his masterpiece from about 1520. One protester, Jessica Agar, a 21-year-old from Hereford, said: "I am an art student but there is no place for me to follow my calling as an artist in a world where I have no future.
"In no uncertain terms, the establishment - of which the Royal Academy is a part - has condemned me and all young people to suffer. I am outraged and you should be too."On Tuesday, protesters attached their own "apocalyptic vision of the future" to the John Constable oil painting the Hay Wain and glued themselves to the frame.in the Suffolk countryside. It is one of the most popular paintings in the National Gallery.
Earlier this week Just Stop Oil allegedly targeted a Scottish art gallery and stormed Sunday's British Grand Prix.
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