New model of multiple sclerosis correlates demyelination with evolution of cognitive and motor abilities InstitutCerveau
Animal care was in accordance with institutional and national guidelines. All animal procedures conformed to the European Community Council 1986 directive as modified in 2010 and have been approved by the ethical committee of the French Ministry of Higher Education and Research .Metronidazole was dissolved in filtered tap water containing 0.1% dimethyl sulphoxide . MTZ was used at concentration of 10 mM with an exposure length of 10 days.
The setup was localized in a darkroom; the light was turned off so that the only light perceived by tadpoles came from the screen. Each animal was tested separately in the Petri dish filled up to 1 cm with MMR 0.1 X medium. Tadpoles were placed in the Petri dish and left to adapt to the screen light for 5–10 s. Spontaneous swimming was recorded for 30 s and average speed for this period analysed.
The virtual avoidance collision test was performed after all animals had been tested for spontaneous swimming behaviour. A black dot was presented on the screen, the experimenter targeted the eye of the tadpole by changing the direction and speed of the dot. On average, 5–6 try outs were performed to assess visual avoidance. The virtual collision setup can be found on the AK websiteAnalysis of videos recordings was with Noldus Ethovision XT 11.5 software.
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