Neonatal mice-based model established that allows for the transmission of clinical SARS-CoV-2 isolates biorxivpreprint nyugrossman UMmedschool SARSCoV2 COVID19 coronavirus covid transmission
By Pooja Toshniwal PahariaOct 7 2022Reviewed by Danielle Ellis, B.Sc. In a recent study posted to the bioRxiv* preprint server, researchers established a neonatal mice model that enabled severe acute respiratory syndrome coronavirus 2 transmission.
About the study In the present study, researchers extended their previous analysis and presented a K18-human angiotensin-converting enzyme 2 -expressing neonate mice model that transmitted SARS-CoV-2 and examined previously and currently circulating SARS-CoV-2 VOC transmission. Immunohistochemistry analysis was performed on the nasopharyngeal tissues of index pups, stained for SARS-CoV-2 nucleocapsid protein. Infectious SARS-CoV-2 particles in retrotracheal lavages and lung homogenates were measured to assess viral tropism for the URT and lower respiratory tract , respectively. The cytokines present in the URT shedding samples of SARS-CoV-2-infected neonatal mice were also analyzed.
By four dpi, SARS-CoV-2 particles were shed by all the contact pups, representing 100% efficiency of WA-1 transmission and SARS-CoV-2 detection in the neonate mice shedding samples indicated robust SARS-CoV-2 infection in the upper airways. In addition, SARS-CoV-2-infected cells were identified in the upper olfactory epithelial cells, indicative of WA-1 replication in the URT.
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