An article in BMCBiology discusses how combining infrared thermography and Eulerian Video Magnification provides a non-invasive method to assess individual animal health in zoos, with great potential to monitor wildlife metabolic indices in situ.
This is the first study to demonstrate that prominent signals corresponding to RR and HR can successfully be extracted from infrared videos coupled with EVM analysis in a variety of species. The combination of IRT and EVM provides a novel tool for both animal care staff in zoological institutions and wildlife researchers.
Fifteen individuals had masses recorded the day of imaging and when a mass estimate wasn’t available, one was used from within 7 days . All procedures were approved by the Cincinnati Zoo and Botanical Garden and Woods Hole Oceanographic Institution Institutional Animal Care and Use Committees and approved by management at collaborating institutions.IRT videos were obtained using the FLIR T540 on a tripod. A scoring system was developed to determine video quality.
]. The video length was extended to 30 s only if no signal was extracted to ensure very low vital rates could be identified. All IRT videos were exported with a black-and-white color palette for consistency. A standardized amplification procedure was used to perform Eulerian video magnification using Lambda vue . For IRT videos, 40 × color magnification was used because pulsation caused a change in temperature, and therefore changes in color. RGB videos were analyzed once with 200 × color magnification and once with 200 × motion magnification to attempt to identify pulsation either by movement within blood vessels or changes in skin color.
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