Scientists uncover a novel cellular mechanism that regulates aging and fertility FORTH_ITE NatureAging
GFP::LGG-1, the GFP puncta area was measured using the maximum pixel intensity of the Z-stacks. In all quantifications, background subtraction and threshold setting were performed.All animal protocols were approved by the FORTH Ethics Committee and the Directorate of Agricultural and Veterinary Political Region of Crete .
Vibrance Antifade Mounting Medium with DAPI was used to mount coverslips on slides and stain nuclei. Confocal images of fluorescently labeled proteins were captured using a 40× objective lens on an LSM 710 NL multi-photon confocal microscope . Puncta number, size and pixel intensity measurements were performed with ImageJ after background subtraction and threshold setting.
Vibrance Antifade Mounting Medium with DAPI. Fluorescent images were acquired using a 60× objective lens of the EVOS FL Auto 2 Cell Imaging System. Images were processed and cropped using ImageJ.
Ireland Latest News, Ireland Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
400 Miles in a 40th Anniversary Toyota 4RunnerAnd back again in this aging but still highly functional SUV.
Read more »
400 Miles in a 40th Anniversary Toyota 4RunnerAnd back again in this aging but still highly functional SUV.
Read more »
Time Spent in Nature Appears to Slow Parkinson's, Alzheimer'sLiving in an area with easy access to parks and rivers appears to slow the progression of devastating neurological diseases, such as Alzheimer's and Parkinson's.
Read more »
Groundwater depletion in California’s Central Valley accelerates during megadrought - Nature CommunicationsLiu et al. used the NASA GRACE/FO missions to show that since 2019, groundwater depletion in California’s Central Valley has accelerated by 31% compared to recent droughts, and has increased by a nearly a factor of 5 compared to the 60-year average.
Read more »
Midway still a mess as Southwest blames Mother Nature on flight cancelationsThe mess at Midway Airport is nowhere near cleaned up, and for many travelers, the nightmare continues.
Read more »
Face of 'Libs of TikTok,' who remained anonymous due to Left's 'violent nature,' finally revealedThe anonymous creator of the viral Twitter account, 'Libs of TikTok,' joined Fox Nation's 'Tucker Carlson Today' to reveal her identity publicly for the first time.
Read more »