Scientists have discovered a molecular pathway essential to long-term social memory in rodents—a finding that could inform research into social disorders such as autism and schizophrenia. SciSignal Neuroscience
In summary, our research elucidates the mechanism of the posttranslational phosphorylation of OXTR at Serand a positive feedback regulation between OXTR and PKD1, which amplifies OXTR signaling and contributes to LTSM consolidation in the MeA. These results provide brain area– and cellular signaling–specific evidence for social memory regulation by the modification of OXTR and insights into the molecular mechanisms underlying social deficits.
The transfection of HEK293A cells was performed with Lipofectamine 2000/3000 . Cells were used 24 to 36 hours after transfection.). Briefly, 20 ng of active PKD1 was resuspended in assay buffer to a volume of 25 ml at a final concentration of 0.8 ng/ml. To initiate the reaction, 10 ml of the phosphorylation mixture was added. The mixture was incubated at 30°C for 30 min, and the reaction was terminated by the addition of SDS–polyacrylamide gel electrophoresis sample buffer.
Tat-OXTR-IL3-S261 and Tat-OXTR-IL3-S261A were synthesized and purified by GL Biochem. The mass and purity of the peptides were verified by high-performance liquid chromatography. The injection dose is 20 μg/μl and 0.5-μl volume each side for rats.Rats or mice were anesthetized with 10% chloral hydrate and briefly perfused transcardially with saline within 15 min, followed by paraformaldehyde in 4% immediately.
After virus injection, rats were implanted with optical fibers. Each fiber was carefully lowered into the MeA . A small amount of denture powder was sprinkled on the surface of the skull, and a small amount of superglue was applied. Then, water and the denture powder were mixed evenly and laid layer upon layer. After surgery, rats were housed individually to allow full recovery. More details of the optical fiber experiment are shown in the fiber photometry analyses below.
Surgery to implant bilateral guide cannulas at the abovementioned coordinates for drug injection was performed under general anesthesia 7 days before the experiments. Injections were performed over 3 min using a Hamilton syringe and a mechanical pump. The injector was left in place for an additional period of 3 min to minimize backflow along the cannula tract.
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