In Alzheimer's disease patients, dentate granule cells show early morphological alterations that are further aggravated as the disease progresses – according to research published in Acta Neuropathologica Communications.
After brain extraction, a mid-sagittal section was made to separate the right and left hemispheres. To dissect the whole hippocampus, the posterior poles of the mammillary bodies and the uncus were first identified []. Afterwards, a coronal 2-cm-thick slice of the whole hemisphere was obtained at this anatomical level.
After a 48-h incubation in solution C at 4 °C, hippocampal tissue blocks were included in a 10% sucrose-4% agarose solution, obtaining 150 µm-thick sections in a Leica VT1200S vibratome. These sections were then mounted on gelatin-coated glass slides and air-dried for ~ 20 min. The Golgi-Cox reaction was then performed following the manufacturer´s instructions. Sections were finally counterstained with toluidine blue to identify anatomical structures.
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