New immunosensor paves the way to quick and sensitive COVID-19 diagnostics tokodai_news
homogeneous immunosensor based on a human anti-N protein antibody. For the first time, we uncovered the crowding agent's role in improving the performance of the double-labeled Quenchbody, and the possible mechanisms behind this improvement are discussed. The 5% polyethylene glycol 6000 significantly improved both the response speed and sensitivity of SARS-CoV-2 Quenchbodies.
The calculated limit of detection for recombinant N protein was 191 pM within 15 min of incubation, which was 9- to 10-fold lower than the assay without adding crowding agent. We also validated the developed immunosensor in a point-of-care test by measuring specimens from COVID-19-positive patients using a compact tube fluorometer.
Ireland Latest News, Ireland Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
What is the relation between COVID-19 vaccination and new POTS-related diagnoses?What is the relation between COVID-19 vaccination and new POTS-related diagnoses? NatureCVR CedarsSinai BrighamWomens COVID19 coronavirus covid vaccination vaccine diagnosis cardiac cardiology coronavirus
Read more »
Bioinformatics approach for the design of siRNAs against SARS-CoV-2Bioinformatics approach for the design of siRNAs against SARS-CoV-2 biorxivpreprint umontpellier RNA siRNA SARSCoV2 COVID19 coronavirus covid bioinformatics
Read more »
Sheep milk could be an inexpensive source of therapeutic polyclonal SARS-CoV-2 neutralizing antibodiesSheep milk could be an inexpensive source of therapeutic polyclonal SARS-CoV-2 neutralizing antibodies biorxivpreprint waikato SARSCoV2 COVID19 Milk SheepsMilk
Read more »
mRNA-FISH for the detection of viral RNA in SARS-CoV-2 infected cells and the determination of the kinetics of early RNA replicationmRNA-FISH for the detection of viral RNA in SARS-CoV-2 infected cells and the determination of the kinetics of early RNA replication biorxivpreprint EinsteinMed IcahnMountSinai RNA SARSCoV2 infection covid coronavirus COVID19
Read more »
Inhibition of glycogen synthase kinase-3-beta (GSK3β) blocks nucleocapsid phosphorylation and SARS-CoV-2 replication - Molecular BiomedicineGSK3β has been proposed to have an essential role in Coronaviridae infections. Screening of a targeted library of GSK3β inhibitors against both SARS-CoV-2 and HCoV-229E to identify broad-spectrum anti-Coronaviridae inhibitors resulted in the identification of a high proportion of active compounds with low toxicity to host cells. A selected lead compound, T-1686568, showed low micromolar, dose-dependent activity against SARS-CoV-2 and HCoV-229E. T-1686568 showed efficacy in viral-infected cultured cells and primary 2D organoids. T-1686568 also inhibited SARS-CoV-2 variants of concern Delta and Omicron. Importantly, while inhibition by T-1686568 resulted in the overall reduction of viral load and protein translation, GSK3β inhibition resulted in cellular accumulation of the nucleocapsid protein relative to the spike protein. Following identification of potential phosphorylation sites of Coronaviridae nucleocapsid, protein kinase substrate profiling assays combined with Western blotting analysis of nine host kinases showed that the SARS-CoV-2 nucleocapsid could be phosphorylated by GSK3β and PKCa. GSK3β phosphorylated SARS-CoV-2 nucleocapsid on the S180/S184, S190/S194 and T198 phospho-sites, following previous priming in the adjacent S188, T198 and S206, respectively. Such inhibition presents a compelling target for broad-spectrum anti-Coronaviridae compound development, and underlies the mechanism of action of GSK3β host-directed therapy against this class of obligate intracellular pathogens.
Read more »
effects of 105 biological, socioeconomic, behavioral, and environmental factors on the risk of SARS-CoV-2 infection and a severe course of COVID-19: a prospective, explorative cohort studyAbstract . The confirmed number of SARS-CoV-2 infections up to 18 October 2022 is 626 million worldwide, but information about factors affecting the probability
Read more »