Rationalized deep learning super-resolution microscopy for sustained live imaging of rapid subcellular processes
. SIM images reconstructed by Hessian-SIM and rDL SIM of CCPs in a SUM159 cell for 149 timepoints at 1-second intervals. The corresponding GT-SIM images are shown for reference. The trajectory of a CCP is delineated to illustrate the temporal degradation effect by Hessian-SIM.Comparison of raw SIM images denoising by deep learning algorithms with and without physical model rationalization
. rDL TIRF-SIM records the adhesion and spreading dynamics of a U2OS cell co-expressing mEmerald-Lifeact and mCherry-myosin-IIA for 749 timepoints at 5-second intervals.. Formation, maturation and internalization of endogenous clathrin-EGFP in a live SUM159 cell for 5,500 timepoints with a time duration of 45.8 minutes at 1-second intervals.
. The spatiotemporal coordination and remodeling of the IFT trains in live mEPCs expressing EGFP-IFT81 and stained by LysoTracker as seen by rDL GI-SIM for 1,946 timepoints at 0.09-second intervals.A set of examples of IFT train transportation and remodeling dynamics along the three groups of MT tracks in the axoneme of live mEPCs
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