Study identifies how diabetes slows healing in the eye
Corneas were organ-cultured on agar-collagen gel in serum-free medium with insulin-transferrin-selenite supplement as previously described [DNA was extracted from LESC-enriched LEC isolated from six non-diabetic donors and five diabetic donors. Bisulphite conversion was carried out according to manufacturer’s instructions .
All computations and statistical analyses were performed using R 2.15.0 and Bioconductor 2.12 .Primary LEC were treated with human recombinant Wnt-5a or DNA methylation inhibitors , or canonical Wnt activator and glycogen synthase kinase -3β inhibitor CHIR99021 . When LEC cultures were 60–80% confluent, they were transfected with 50 nmol/l ofor negative control siRNAs , or hsa-miR-203a mimic and inhibitor with their respective mimic or inhibitor controls, using Lipofectamine RNAiMAX .
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