SARS-CoV-2 RNA found to be present in various environmental surface samples unevadareno COVID19 SARSCoV2 RNA Surface Samples
By Tarun Sai LomteNov 29 2022Reviewed by Aimee Molineux In a recent study published in PLOS ONE, researchers conducted environmental surveillance of severe acute respiratory syndrome coronavirus 2 ribonucleic acid .
About the study In the present study, researchers performed the environmental surface sampling of SARS-CoV-2 RNA in select high-traffic public places and a public health facility in Las Vegas, the United States . Three hundred surface samples were collected between December 7, 2020, and April 22, 2021. from frequently touched surfaces in public locations, such as post offices, gas stations, car washes, shopping malls, and grocery store restrooms.
Findings Related StoriesThe authors collected 150 samples from public locations and 150 from the PHF. Overall, 31 were positive for SARS-CoV-2 RNA. Of these, 24 PHF samples and seven specimens from high-traffic public areas were positive for viral RNA. The seven positive specimens were collected from a handrail, door handle, elevator buttons, crosswalk pedestrian push buttons, sinks, toilet seats, and doorknobs of restrooms.
One-way analysis of variance showed significant differences between the average Ct values of the three assays. It revealed that the N gene assay was more sensitive than the ORF/S gene assay. The detection limit was 86 – 860 SARS-CoV-2 RNA copies/sample, equivalent to 3 to 33 RNA copies/cm2.
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