A soybean protein blocks LDLcholesterol production, reducing risks of metabolic diseases UofIllinois
2.8.6. Cholesterol Metabolism-Related Protein Expression by Western Blot
Similar quantities of cell lysate protein were separated by electrophoresis using 4–20% gradient SDS-PAGE gels. Separated proteins were transferred onto PVDF membranes , which were then blocked with 5% nonfat dry milk in Tris-buffered saline + 0.1% Tween 20 for 1 h at room temperature. Membranes were incubated overnight at 4 °C with primary mouse antibodies for human p-AMPK, ACC , SREBP-1c , FASN , LDLR , and PCSK9 purchased in Santa Cruz Biotechnology, unless otherwise stated.
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Edinburgh scientists work on restoring whisky flavoursResearchers will test 200-year-old barley varieties to see how they react to modern processing.
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Sheffield: Heritage orchard to preserve rare local fruit treesA new heritage orchard in Sheffield aims to rescue rare local fruit varieties.
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Scientists explore synthesis of griffithsin in cell-free systemsScientists explore synthesis of griffithsin in cell-free systems biorxivpreprint UMBC Synthesis Griffithsin CellFreeSystems
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