Nature research paper: Structure deformation and curvature sensing of PIEZO1 in lipid membranes
, Cryo-EM micrograph of empty liposomes prepared on the Quantifoil Au grid ., Analyses of the radii of the PIEZO1-residing side and the opposite pole of the 2D averaged proteoliposome vesicles of varied sizes. The inner and outer bilayers are approximated with the red and yellow dashed circles that are based on the PIEZO1-residing pole and the opposite pole of the same vesicle, respectively.
Extended Data Fig. 3 Structural determination and analyses of the curved PIEZO1 structure derived from proteoliposomes., 2D class averages of PIEZO1 proteoliposomes with a circular mask of 25 nm diameter, showing the cap domain located inside the vesicles. On the basis of the abruptly changed curvature, the red arrows might indicate the boundary between the PIEZO1-residing side and the opposite side lacking proteins., Gold-standard Fourier shell correlation curve of the indicated density map.
, The cartoon model of the curved PIEZO1 structure derived from proteoliposomes. The featured structural domains are labeled. The resolved blade lipids and pore lipids are shown in blue and green, respectively., Pore radius along the central axis of the ion conduction pathway of the indicated PIEZO1 structures derived from either detergent micelles or the curved structure derived from proteoliposomes. The residues forming the TM gate and intracellular constriction neck are labeled.
Extended Data Fig. 4 Flowchart of EM data processing of the proteoliposomes with PIEZO1 being reconstituted in the outside-in configuration.Extended Data Fig. 5 Local EM density of the indicated domains of the curved PIEZO1 structure derived from proteoliposomes., The helices are shown in cartoon representation with side chains as sticks. The cryo-EM density is shown as gray mesh.
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