The evolutionary role of actin within sophisticated architectures of biosilica in sponges NatureChemistry advscinews
, Ehrlich et al. identified actin in the axial filaments of a variety of sponges.
Identification of actin in the axial filament of the glass sponge Asconema setubalense. a) iFluor 594-Phalloidin red staining of partially demineralized spicules shows the axial filament. b) The amino acid sequence of actin was identified by the MS proteomic approach in the axial filament of A. setubalense. Seven peptides were identified using an in-solution digestion and label-free nanoLC-MS/MS approach , yielding 27.1% coverage of a protein sequence . c) SDS-PAGE analysis of the A.
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