A study published in Respiratory Research evaluates the role of nestin, a type VI intermediate filament protein, in cytoskeletal signaling in airway smooth muscle.
]. Again, human tissues were non-transplantable, and informed consents were obtained from all subjects for research. This study was approved by the Albany Medical College Committee on Research Involving Human Subjects. Bronchi were slowly filled with warm 2% low-melting-point agarose prepared in Hank’s Balanced Salt Solution . Once the lungs were filled with agarose, bronchi were clamped to prevent leakage.
were placed in the modified physiological saline solution for 30 min in an incubator and subsequently placed in a 35-mm glass bottom culture dish with the modified physiological saline solution.
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