Jumping gene found to be strongly linked to depression, fear and anxiety oistedu
gene with detailed procedure described in supplementary information. Genotyping to detect insertion at the 5’ end employed primers: FW 5’- TGAGAGCCCTTGGCATGG -3’ REV 5’- ATACCACTTCCCAGCAGG -3’and at 3’ end using: FW 5’- GGAATAATGGAAGGCAGG -3’ REV 5’- CCTCCTATCACCTGGCTC -3’. Mice with homozygous LoxP insertions were used for experiments after backcrossing with C57BL/6 J mice for at least 5 generations. All mice were housed under controlled temperature and a 12-h light/dark cycle.
Synaptic fractionation was done following a detailed published protocol []. Electrophoresis was performed using 7.5 or 12% TGX Acrylamide gels following standard protocols. Proteins were transferred to PVDF membranes using Trans-Blot Turbo Transfer and blocked in TBS buffer containing 5% BSA and 0.1% Tween-20. Antibodies were diluted in Can Get Signal immunoreaction enhancer solution and incubated according to the manufacturer’s protocol.
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