Medicines that modify the circadianclock might help heal scars more cleanly frontiersin
expression data were uploaded to an online data analysis tool , on which data were normalized and themight be false positives due to cytotoxicity that leads to cell death or suppresses growth in cells that have not been suppressed. In order to prevent this problem, separate high-throughput screening for cell viability was conducted using the same FDA-approved drug library.
For the purposes of this experiment, media changes with AA supplementation were conducted every 3 days over a course of 14 days, with each media change coinciding with a simultaneous hit supplementation by a single hit compound at the specified dosage for that treatment group. This method was used to study the effect of each hit compound at the specified concentration throughout the course of normal wound healing.
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Frontiers | Spatiotemporal local and abscopal cell death and immune responses to histotripsy focused ultrasound tumor ablationHistotripsy is a novel focused ultrasound tumor ablation modality with potent immunostimulatory effects. To measure the spatiotemporal kinetics of local and abscopal responses to histotripsy, C57BL/6 mice bearing bilateral flank B16 melanoma or Hepa1-6 hepatocellular carcinoma tumors were treated with unilateral sham or partial histotripsy. Treated and contralateral untreated (abscopal) tumors were analyzed using multicolor immunofluorescence, digital spatial profiling, RNA sequencing (RNASeq), and flow cytometry. Unilateral histotripsy triggered abscopal tumor growth inhibition. Within the ablation zone, early high mobility group box protein 1 (HMGB1) release and necroptosis were accompanied by immunogenic cell death transcriptional responses in tumor cells and innate immune activation transcriptional responses in infiltrating myeloid and natural killer (NK) cells. Delayed CD8+ T cell intratumoral infiltration was spatiotemporally aligned with cancer cell features of ferroptosis; this effect was enhanced by CTLA-4 blockade and recapitulated in vitro when tumor-draining lymph node CD8+ T cells were co-cultured with tumor cells. Inoculation with cell-free tumor fractions generated by histotripsy but not radiation or freeze/thaw conferred partial protection from tumor challenge. We propose that histotripsy may evoke local necroptotic immunogenic cell death, priming systemic adaptive immune responses and abscopal ferroptotic cancer cell death.
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Avatar: The Way of Water sucks, but it made me really excited about Ubisoft's upcoming gameAvatar: Frontiers of Pandora could be brilliant if it leans into the universe's many oddities.
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Frontiers | Preventing stillbirth from obstructed labor: A sensorized, low-cost device to train in safer operative birthBackground: 98% of stillbirths occur in low- and middle- income countries. Obstructed labor is a common cause for both neonatal and maternal mortality, with a lack of skilled birth attendants one of the main reasons for the reduction in operative vaginal birth, especially in low- and middle- income countries. We introduce a low cost, sensorized, wearable device for digital vaginal examination to facilitate accurate assessment of fetal position and force applied to the fetal head, to aid training in safe operative vaginal birth. Methods: The device consists of flexible pressure/force sensors mounted onto the fingertips of a surgical glove. Phantoms of the neonatal head were developed to replicate sutures. An Obstetrician tested the device on the phantoms by performing a mock vaginal examination at full dilatation. Data was recorded and signals interpreted. Software was developed so that the glove can be used with a simple smartphone app. A patient and public involvement panel was consulted on the glove design and functionality. Results: The sensors achieved a 20 Newton force range and a 0.1 Newton sensitivity, leading to 100% accuracy in detecting fetal sutures, including when different degrees of molding or caput were present. They also detected sutures and force applied with a second sterile surgical glove on top. The software developed allowed a force threshold to be set, alerting the clinician when excessive force is applied. Patient and public involvement panels welcomed the device with great enthusiasm. Feedback indicated that women would accept, and prefer, clinicians to use the device if it could improve safety and reduce the number of vaginal examinations required. Conclusion: Under phantom conditions to simulate the fetal head in labor, the novel sensorized glove can accurately determine fetal sutures and provide real-time force readings, to support safer clinical training and practice in operative birth. The glove is low cost (approximately 1 USD). Softwar
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Surrey dog-walker Natasha Johnston died from multiple dog bites to her neck, inquest hearsDog walker, 28, died from multiple dog bites, an inquest opening into her death has heard Natasha Johnston was attacked on 12 January at a beauty spot in Caterham, Surrey. 🔴 joeduggan1 reports
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Man yelled 'I'm from Manchester' before he was murdered by 'mob', court hearsMan yelled 'I'm from Manchester' before he was murdered by 'out of control mob', court hears
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Two men charged with murder of council worker shot dead in gardenBREAKING: James Witham and Joseph Peers have been charged with the murder of council worker Ashley Dale after she was found dead with a gunshot wound in her back garden.
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