A study published in Molecular Medicine finds that homocysteine accelerates atherosclerosis progression by enhancing macrophages pyroptosis via promoting endoplasmic reticulum (ER) stress, ER-mitochondria coupling, and calcium disorder.
In the present study, we used an atherosclerotic mouse model to investigate the role Hcy plays in regulating macrophage pyroptosis during the progression of atherosclerotic plaques. Further, we aimed to elucidate the underlying molecular mechanisms behind this process.Human monocytic THP-1 cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum .
humidified atmosphere. Human monocyte-derived macrophages were treated with 100 nM phorbol myristate acetate for 24 h to obtain macrophages. After pretreatment with inhibitor VX765 or NAC or 4PBA or BAPTA for 1 h, the cells were treated with 10 μM Hcy for 24 h.Total intracellular RNA was extracted and purified by Trizol reagent according to manufacturer’s instructions.
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Yes, That Was A Certain Acting Legend You Spotted In The Background Of The Partygate DebateStill not the strangest celebrity sighting at the House Of Commons this month, though.
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Molecular mechanisms underlying the high mortality of hypervirulent Klebsiella pneumoniae and its effective therapy development - Signal Transduction and Targeted TherapySignal Transduction and Targeted Therapy - Molecular mechanisms underlying the high mortality of hypervirulent Klebsiella pneumoniae and its effective therapy development
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The intermediate filament protein nestin serves as a molecular hub for smooth muscle cytoskeletal signaling - Respiratory ResearchBackground The recruitment of the actin-regulatory proteins cortactin and profilin-1 (Pfn-1) to the membrane is important for the regulation of actin cytoskeletal reorganization and smooth muscle contraction. Polo-like kinase 1 (Plk1) and the type III intermediate filament protein vimentin are involved in smooth muscle contraction. Regulation of complex cytoskeletal signaling is not entirely elucidated. The aim of this study was to evaluate the role of nestin (a type VI intermediate filament protein) in cytoskeletal signaling in airway smooth muscle. Methods Nestin expression in human airway smooth muscle (HASM) was knocked down by specific shRNA or siRNA. The effects of nestin knockdown (KD) on the recruitment of cortactin and Pfn-1, actin polymerization, myosin light chain (MLC) phosphorylation, and contraction were evaluated by cellular and physiological approaches. Moreover, we assessed the effects of non-phosphorylatable nestin mutant on these biological processes. Results Nestin KD reduced the recruitment of cortactin and Pfn-1, actin polymerization, and HASM contraction without affecting MLC phosphorylation. Moreover, contractile stimulation enhanced nestin phosphorylation at Thr-315 and the interaction of nestin with Plk1. Nestin KD also diminished phosphorylation of Plk1 and vimentin. The expression of T315A nestin mutant (alanine substitution at Thr-315) reduced the recruitment of cortactin and Pfn-1, actin polymerization, and HASM contraction without affecting MLC phosphorylation. Furthermore, Plk1 KD diminished nestin phosphorylation at this residue. Conclusions Nestin is an essential macromolecule that regulates actin cytoskeletal signaling via Plk1 in smooth muscle. Plk1 and nestin form an activation loop during contractile stimulation.
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Associations between smoking and vaping prevalence, product use characteristics, and mental health diagnoses in Great Britain: a population survey - BMC MedicineBackground Rates of diseases and death from tobacco smoking are substantially higher among those with a mental health condition (MHC). Vaping can help some people quit smoking, but little is known about vaping among people with MHCs or psychological distress. We assessed the prevalence and characteristics (heaviness, product type) of smoking and/or vaping among those with and without a history of single or multiple MHC diagnoses and with no, moderate or serious psychological distress. Methods Data from 27,437 adults in Great Britain surveyed between 2020 and 2022. Multinomial regressions analysed associations between smoking, vaping and dual use prevalence, smoking/vaping characteristics and (a) history of a single or multiple MHC and (b) moderate or serious psychological distress, adjusted for age, gender, and socioeconomic status. Results Compared with people who had never smoked, those who currently smoked were more likely to report a history of a single (12.5% vs 15.0%, AOR=1.62, 95% CI=1.46–1.81, p|.001) or multiple MHCs (12.8% vs 29.3%, AOR=2.51, 95% CI=2.28–2.75, p|.001). Compared with non-vapers, current vapers were more likely to report a history of a single (13.5% vs 15.5%, AOR=1.28, 95% CI=1.11–1.48, p|.001) or multiple MHCs (15.5% vs 33.4%, AOR=1.66, 95% CI=1.47–1.87, p|.001). Dual users were more likely to report a history of multiple MHCs (36.8%), but not a single MHC than exclusive smokers (27.2%) and exclusive vapers (30.4%) (all p|.05). Similar associations were reported for those with moderate or serious psychological distress. Smoking roll-your-own cigarettes and smoking more heavily, were associated with a history of single or multiple MHCs. There were no associations between vaping characteristics and a history of MHCs. Frequency of vaping, device type and nicotine concentration differed by psychological distress. Conclusions Smoking, vaping and dual use were substantially higher among those with a history of MHC, especially multiple MHC, and ex
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UCLan medical students to benefit from new surgeons agreementThe city's university has been ramping up what it provides in terms of medical courses in recent years - and there's another step forward here at uclan 🧑⚕️🧑⚕️🧑⚕️
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