How useful is PCR for diagnosing monkeypox? Monkeypox PCR Diagnosis Diagnostics Eurosurveillanc NML_ChemBioGC UniOfSurrey bengurionu ucl uclh
By Dr. Liji Thomas, MDAug 16 2022Reviewed by Benedette Cuffari, M.Sc. The World Health Organization declared the recent outbreak of monkeypox virus infections in non-endemic regions a public health emergency of international concern on July 23, 2022. This has led public health officials in many countries to define guidelines on the early detection, case isolation, and containment measures to mitigate the spread of this virus.
Contamination may rise due to contamination of negative samples from positive samples with a high viral load when both are in the same run. Contamination may also alter the integrity of synthetic templates like amplicons, which is why specimens are prepared separately from the site of analysis. With increased surveillance, genetic changes that may affect test performance and increase false-negative results should be identified.
The preferred method for quantification is measuring the number of units of the viral genome in copies per unit volume. This requires the use of normalized standards that have undergone a full characterization and are, as a result, reliable for this type of test evaluation.
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